PROCEEDINGS OF THE SHEVCHENKO SCIENTIFIC SOCIETY

Chemical Sciences

Archive / Volume LXXIII 2023

Dmytro POLOVYI1, Natalia DEMIANIUK1, Anna DEMYD1, Larisa BOYKO1, Hryhoriy ZAGRYCHUK1, Olena AKSIMENTYEVA-KRASNOPOLSKA2

1I. Horbachevsky Ternopil National Medical University St. Ruska, 36, 46001 Ternopil, Ukraine
e-mail: dpolov1981@gmail.com

2Ivan Franko National University of Lviv , Kyryla i Mephodia Str., 6, 79005 Lviv, Ukraine
e-mail: aksimen@ukr.net

DOI: https://doi.org/10.37827/ntsh.chem.2023.73.136

SPECTROPHOTOMETRIC DETERMINATION OF PHENOLIC DERIVATIVES IN PLANT EXTRACTS

For centuries, plants have been used as a source of medicine to treat infections, cardiovascular disease, cancer, or as immune suppressants. The active component of aqueous extracts of plant parts in many cases are phenolic derivatives. Their quantitative determination according to the pharmacopoeia is carried out by the method of thin-layer chromatography and colorimetry. The paper proposes a spectrophotometric study of plant extracts using a UNICO-1200 spectrophotometer. In order to speed up the analysis process, it is proposed to make changes to the software control of the device, to add items to the user menu that allow automating the analysis process and calculating the content of phenolic derivatives in the analyzed raw materials. To improve the capabilities of the spectrophotometer, the microcontroller was replaced with a more productive 32-bit STM32F103, and a program code was developed for it. This allows not only to improve the technical characteristics of the device, but also to create new work algorithms.
In this definition, instead of constructing a calibration graph, the addition method is used. Acetate buffer solution (pH=4.8) and ferric chloride solution are added to an aliquot of aqueous extract prepared from a weight of dry plant material. The software of the spectrophotometer independently determines the absorption maximum of the phenol complex of ferrum. The analytical signal is a maximum in the range of 534 - 696 nm. The deviation of the position of the absorption maximum does not exceed 2%. With the help of menu items, enter such data as the volumes of aliquots, the volumes of flasks, the weight of dry plant material and the volume of water for preparing the extract, the mass of the standard substance that was added as an analytical additive. After entering all the data, the result of the analysis appears on the scoreboard in the form of the mass of phenolic derivatives in terms of the standard substance.
In the future, the obtained results may form the basis of an improved technique for the spectrophotometric determination of phenolic derivatives by the additive method.

Keywords: spectrophotometry, phenol derivatives, plant extract, microcontroller, optical density, ferrum complexes, additive method.

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How to Cite

POLOVYI D., DEMIANIUK N., DEMYD A., BOYKO L., ZAGRYCHUK H., AKSIMENTYEVA-KRASNOPOLSKA O. SPECTROPHOTOMETRIC DETERMINATION OF PHENOLIC DERIVATIVES IN PLANT EXTRACTS. Proc. Shevchenko Sci. Soc. Chem. Sci. 2023. Vol. LXXIII. P. 136-143.

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